Overcoming cross-incompatibility between Nicotiana tabacum L. and N. rustica L. by test-tube pollination and ovule culture.
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چکیده
منابع مشابه
Coproporphyrinogenase in tobacco (Nicotiana tabacum L.).
1. Coproporphyrinogenase was extracted and purified from tobacco (Nicotiana tabacum L.). Enzyme activity was mainly located in mitochondria rather than in chloroplasts. The enzyme was purified by differential centrifugation, ammonium sulphate fractionation, calcium phosphate gel adsorption and dialysis. A 69-fold final purification was obtained. 2. An apparent K(m) value of 3.6x10(-5)m was foun...
متن کاملGC-MS and MALDI-TOF MS profiling of sucrose esters from Nicotiana tabacum and N. rustica.
Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been applied for the first time to the analysis of the sucrose esters from the surface of Nicotiana L. leaves. The profiles obtained for the model plant N. tabacum were similar to those from the gas chromatography-flame ionization detector (GC-FID) analysis. The most reproducible results were obtaine...
متن کاملCarbohydrate-limited Growth Kinetics of Tobacco (Nicotiana rustica L.) Callus.
Logistic curves were fitted to sigmoidal growth data obtained from tobacco (Nicotiana rustica L.) callus grown on media prepared with 0.1, 0.03, 0.01, and 0.003 m sucrose. Analysis of the growth curves indicated that final yields and specific growth rates were influenced by the initial sucrose concentration. Growth yields from the four treatments were similar (0.61 +/- 0.04 gram dry tissue per ...
متن کاملTobacco (Nicotiana tabacum L.)-A model system for tissue culture interventions and genetic engineering
Tobacco (Nicotiana tabacum L.) has become a model system for tissue culture and genetic engineering over the past several decades and continues to remain the 'Cinderella of Plant Biotechnology', An ill vitro culture medium (Murashige and Skoog, 1962), based on the studies with tobacco tissue cultures, has now been widely used as culture medium formulation for hundreds of plant species. Studies ...
متن کاملFlower Variegation in Hybrids between NICOTIANA TABACUM and N. OTOPHORA.
HE fate of genes and chromosomes following interspecific gene transfer Tdeserves detailed study. Genes of one species often can be introduced into another without evident changes of their action and with little effect upon their stability. ANDERSON'S (1949) widely known work on introgression after interspecific hybridization could not have been successful had he been dealing with unstable genet...
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ژورنال
عنوان ژورنال: Japanese Journal of Breeding
سال: 1985
ISSN: 0536-3683,2185-291X
DOI: 10.1270/jsbbs1951.35.429